New Aslef strike action planned for the end of January into early February could cost hospitality businesses almost £350m, according to UKHospitality.
Aslef announced another series of strikes between 30 January and 5 February, affecting different train operators each day.
Furthermore, an overtime ban will also be in place from 29 January to 6 February.
The latest disruption will be another blow to venues, which have already lost more than £4bn in sales from rail strikes.
Kate Nicholls, chief executive of UKHospitality, said: “Once again, hospitality businesses are left dismayed by strike action which will be significantly detrimental to sales and see bookings cancelled. January and February are already two of the quieter months of the year for venues and this disruption will make it even more painful.
“We estimate this set of strikes will cost hospitality almost £350m, on top of the £4bn in lost sales the sector has already had to withstand. We continue to urge all parties involved to urgently reach an agreement and resolve this dispute. Ongoing strike action hurts businesses, prevents people from getting to work and significantly erodes confidence in the rail network.”
This news comes after planned industrial action by the RMT last week was called off as a resolution was reached.